Monday, December 21, 2020

IBEX: A New Open-Source Method for Multiplex Tissue Imaging

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Monday, December 21, 2020

IBEX: A New Open-Source Method for Multiplex Tissue Imaging

Confocal images from IBEX experiments with various mouse organs

Confocal images from IBEX experiments with various mouse organs. Credit: NIAID

Dr. Andrea Radtke and colleagues in Dr. Ronald Germain's laboratory at NIAID have developed a new open-source method for highly multiplex tissue imaging called Iterative Bleaching Extends multi-pleXity, or IBEX. This broadly applicable method uses conventional microscopes to obtain high-dimensional imaging data to reveal the complex cellular landscape of diverse organs and tissues, the scientists explain in Proceedings of the National Academy of Sciences. Dr. Radtke and colleagues also present extensions of the IBEX workflow to handle hard-to-image tissue preparations and a method to facilitate direct integration of imaging data with flow cytometry and sequencing technologies.

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